Growth Phase Regulation of flaA Expression in Helicobacter pylori Is luxS Dependent

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Growth phase regulation of flaA expression in Helicobacter pylori is luxS dependent.

LuxS plays a role in the synthesis of an extracellular signaling molecule, autoinducer 2 (AI-2). To analyze a possible role of AI-2 in regulating Helicobacter pylori gene expression, we constructed a panel of transcriptional reporter strains. We show that the expression of H. pylori flaA is growth phase dependent and that flaA transcription increases in association with increased culture densit...

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Antigenic and immunogenic evaluation of Helicobacter pylori FlaA epitopes

Objective(s): Helicobacter pyloriare among most common human pathogens affecting at least half of the world’s population. Mobility is one of the important primary factors in bacterial colonization and invasion. The purpose of this research is cloning, expression, and purification of FlaA protein specific epitopes in order to evaluate their antigenicity and immunogenicity. Materials and Methods:...

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Balancing metal uptake is essential for maintaining a proper intracellular metal concentration. Here, we report the transcriptional control exerted by the two metal-responsive regulators of Helicobacter pylori, Fur (iron-dependent ferric uptake regulator) and NikR (nickel-responsive regulator), on the three copies of the fecA genes present in this species. By monitoring the patterns of transcri...

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Antigenic and immunogenic evaluation of Helicobacter pylori FlaA epitopes

OBJECTIVES Helicobacter pylori are among most common human pathogens affecting at least half of the world's population. Mobility is one of the important primary factors in bacterial colonization and invasion. The purpose of this research is cloning, expression, and purification of FlaA protein specific epitopes in order to evaluate their antigenicity and immunogenicity. MATERIALS AND METHODS ...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 2004

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.72.9.5506-5510.2004